Prod.-No.DescriptionInhibitor ClassTarget Enzyme
Recommended Working ConcentrationStock Solution
A1421 AEBSF Serine proteases Thrombin, Chymotrypsin, Kallikrein, Plasmin, Proteinase K, Trypsin 0.1 - 2 mM 20 - 100 mM in buffer
A3764 Amastatin Amino peptidases Aminopeptidase A, Leucine aminopeptidase, many membrane-bound peptidases    
A2126 p-Aminobenzamidine Serine proteases Trypsin, Plasmin, Thrombin 1 mM 100 mM in water
A2266 epsilon-Aminocaproic acid Serine proteases   5 mM 500 mM inbuffer
A2129 Antipain Serine/Cysteine proteases Trypsin, Papain, Cathepsin B 10 - 50 µg/ml 10 mg/ml in water, DMSO, MeOH
A0998 APMSF Serine proteases Trypsin, Thrombin, Factor Xa, Plasmin 10 - 50 µM 1 - 5 mM in water
A2132 Aprotinin Serine proteases Trypsin, Chymotrypsin, Kallikrein, Plasmin 2 - 10 µg/ml 10 mg/ml in water
A1380 Benzamidine Serine proteases Trypsin, Thrombin, Plasmin 1 - 5 mM up to 1 M in water
A2137 Bestatin Amino peptidases Aminopeptidase B, Leucine aminopeptidase, Tripeptide aminopeptidase, Aminopeptidases of the cell surface 40 µg/ml (approx. 130 µM) 1 - 5 mg/ml in MeOH
A2144 Chymostatin Serine/Cysteine proteases α-, β-, γ-, δ-Chymotrypsin, Papain, Cathepsin A, B and D 6 - 60 µg/ml
(10 - 100 µM)
20 mg/ml in DMSO, acetic acid
A2157 E-64 Cysteine proteases Papain, Bromelain, Calpain, Cathepsin B, H, L, Tumor-Cathepsin, Streptococcus-Protease, Ficin 10 µM 1 - 10 mM in DMSO, 50 % EtOH
A1104 EDTA Metallo proteases   1 - 10 mM 500 mM in water
A0878 EGTA Metallo proteases KEX 2, Calcium-dependent proteases 1 - 10 mM in aqueous solution
A1666 Iodoacetamide $ Serine proteases Cerastocytin 1 - 5 mM
(185 - 925 µg/ml)
in aqueous solution
A2097 Iodoacetic acid $ Serine proteases   1 - 5 mM
(208 - 1040 µg/ml)
in aqueous solution
A2183 Leupeptin Serine/Cysteine proteases Plasmin, Trypsin, Papain, Cathepsin B, Thrombin, Calpain 5 - 50 µg/ml
(10 - 100 µM)
1 mg/ml in aqueous solutions
A2186 α2-Macroglobulin many Endoproteases Carboxyl-, Thiol-, Serine , Metalloproteases   5 - 10 mg/ml in buffer
A2205 Pepstatin A acidic proteases, Aspartic proteases Pepsin, Cathepsin D, Renin, HIV- and MMTV-Protease 1 - 5 µM
(0.7 - 3.5 µg/ml)
in MeOH, EtOH, acetic acid, DMSO
A3826 1,10-Phenanthroline Metallo proteases Carboxypeptidase G2 from Pseudomonas; Peptidase T and Acetylornithinase from E. coli; Aminopeptidases from Apis mellifera; Peptidase V from L. delbrueckii etc. 1 - 200 µg/ml
(0.5 µM - 1 mM)
or up to 2 mg/ml (10 mM)
in EtOH, acetone
A2214 Phosphoramidon Metallo proteases Thermolysin, Endothelin-converting enzyme 1 - 10 µM in water, MeOH
A0999 PMSF * Serine/Cysteine proteases Trypsin, Chymotrypsin, Thrombin, Papain 0.1 - 1 mM 10 - 100 mM in EtOH
(1.74 - 17.4 mg/ml)
A1799 TLCK Serine proteases Trypsin, Cerastocytin, not Chymotrypsin 50 µg/ml 1 - 20 mg/ml in buffer
pH <= 6
A1801 TPCK Serine/Cysteine proteases Chymotrypsin, Cerastocytin, Papain, Ficin, not Trypsin 10 - 100 µg/ml
28.4 - 284 µM
10 mg/ml in EtOH, MeOH
A1441 Trypsin inhibitor §   Trypsin, Chymotrypsin, Plasmin, Thrombin, Plasma Kallikrein 10 - 100 µg/ml 1 mg/ml in water

Legend

$ Inactivation by reducing agents

§ from soybean

* The inhibition of cysteine proteases can be reverted by reducing thioles (e.g. DTT).

Abbreviations

AEBSF (4-(2-Aminoethyl)-benzolsulfonylfluoride); APMSF (4-Amidinophenylmethanesulfonylfluoride); E-64 (N-(trans-Epoxysuccinyl)-L-leucine-4-guanidinobutylamide); EDTA (Ethylenediaminetetraacetate); EGTA (Ethyleneglycol-bis-(2-aminoethyl)-tetraacetate); EtOH (Ethanol); MeOH (Methanol)PMSF (Phenylmethanesulfonylfluoride); TLCK (Nα-Tosyl-L-lysine chloromethylketone); TPCK (Tosyl-L-phenylalanine chloromethylketone)

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